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Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions

Overview of attention for article published in Nature Communications, February 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (73rd percentile)

Mentioned by

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7 X users
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1 patent
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1 Facebook page

Citations

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75 Dimensions

Readers on

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109 Mendeley
Title
Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions
Published in
Nature Communications, February 2017
DOI 10.1038/ncomms14472
Pubmed ID
Authors

Andreas Sprengel, Pascal Lill, Pierre Stegemann, Kenny Bravo-Rodriguez, Elisa-C. Schöneweiß, Melisa Merdanovic, Daniel Gudnason, Mikayel Aznauryan, Lisa Gamrad, Stephan Barcikowski, Elsa Sanchez-Garcia, Victoria Birkedal, Christos Gatsogiannis, Michael Ehrmann, Barbara Saccà

Abstract

The self-organizational properties of DNA have been used to realize synthetic hosts for protein encapsulation. However, current strategies of DNA-protein conjugation still limit true emulation of natural host-guest systems, whose formation relies on non-covalent bonds between geometrically matching interfaces. Here we report one of the largest DNA-protein complexes of semisynthetic origin held in place exclusively by spatially defined supramolecular interactions. Our approach is based on the decoration of the inner surface of a DNA origami hollow structure with multiple ligands converging to their corresponding binding sites on the protein surface with programmable symmetry and range-of-action. Our results demonstrate specific host-guest recognition in a 1:1 stoichiometry and selectivity for the guest whose size guarantees sufficient molecular diffusion preserving short intermolecular distances. DNA nanocontainers can be thus rationally designed to trap single guest molecules in their native form, mimicking natural strategies of molecular recognition and anticipating a new method of protein caging.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 109 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 <1%
Unknown 108 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 27%
Researcher 17 16%
Student > Master 14 13%
Student > Bachelor 6 6%
Student > Postgraduate 4 4%
Other 16 15%
Unknown 23 21%
Readers by discipline Count As %
Chemistry 25 23%
Biochemistry, Genetics and Molecular Biology 23 21%
Agricultural and Biological Sciences 12 11%
Engineering 8 7%
Materials Science 7 6%
Other 8 7%
Unknown 26 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2019.
All research outputs
#4,463,910
of 22,953,506 outputs
Outputs from Nature Communications
#31,721
of 47,245 outputs
Outputs of similar age
#79,882
of 307,002 outputs
Outputs of similar age from Nature Communications
#645
of 908 outputs
Altmetric has tracked 22,953,506 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 47,245 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.8. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,002 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 908 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.