Title |
Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions
|
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Published in |
Nature Communications, February 2017
|
DOI | 10.1038/ncomms14472 |
Pubmed ID | |
Authors |
Andreas Sprengel, Pascal Lill, Pierre Stegemann, Kenny Bravo-Rodriguez, Elisa-C. Schöneweiß, Melisa Merdanovic, Daniel Gudnason, Mikayel Aznauryan, Lisa Gamrad, Stephan Barcikowski, Elsa Sanchez-Garcia, Victoria Birkedal, Christos Gatsogiannis, Michael Ehrmann, Barbara Saccà |
Abstract |
The self-organizational properties of DNA have been used to realize synthetic hosts for protein encapsulation. However, current strategies of DNA-protein conjugation still limit true emulation of natural host-guest systems, whose formation relies on non-covalent bonds between geometrically matching interfaces. Here we report one of the largest DNA-protein complexes of semisynthetic origin held in place exclusively by spatially defined supramolecular interactions. Our approach is based on the decoration of the inner surface of a DNA origami hollow structure with multiple ligands converging to their corresponding binding sites on the protein surface with programmable symmetry and range-of-action. Our results demonstrate specific host-guest recognition in a 1:1 stoichiometry and selectivity for the guest whose size guarantees sufficient molecular diffusion preserving short intermolecular distances. DNA nanocontainers can be thus rationally designed to trap single guest molecules in their native form, mimicking natural strategies of molecular recognition and anticipating a new method of protein caging. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Germany | 3 | 43% |
United States | 1 | 14% |
Unknown | 3 | 43% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 6 | 86% |
Scientists | 1 | 14% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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France | 1 | <1% |
Unknown | 108 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 29 | 27% |
Researcher | 17 | 16% |
Student > Master | 14 | 13% |
Student > Bachelor | 6 | 6% |
Student > Postgraduate | 4 | 4% |
Other | 16 | 15% |
Unknown | 23 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 25 | 23% |
Biochemistry, Genetics and Molecular Biology | 23 | 21% |
Agricultural and Biological Sciences | 12 | 11% |
Engineering | 8 | 7% |
Materials Science | 7 | 6% |
Other | 8 | 7% |
Unknown | 26 | 24% |