| Title |
A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking
|
|---|---|
| Published in |
Nature Chemistry, June 2013
|
| DOI | 10.1038/nchem.1706 |
| Pubmed ID | |
| Authors |
Si Kyung Yang, Xinghua Shi, Seongjin Park, Taekjip Ha, Steven C. Zimmerman |
| Abstract |
Single-molecule fluorescence techniques have emerged as a powerful approach to understanding complex biological systems. However, a challenge researchers still face is the limited photostability of nearly all organic fluorophores, including the cyanine and Alexa dyes. We report a new, monovalent probe that emits in the far-red region of the visible spectrum with properties desirable for single-molecule optical imaging. This probe is based on a ring-fused boron-dipyrromethene (BODIPY) core that is conjugated to a polyglycerol dendrimer (PGD). The dendrimer makes the hydrophobic fluorophore water-soluble. This probe exhibits excellent brightness, with an emission maximum of 705 nm. We have observed strikingly long and stable emission from individual PGD-BODIPY probes, even in the absence of anti-fading agents such as Trolox, a combined oxidizing-reducing agent often used in single-molecule studies for improving the photostability of common imaging probes. These interesting properties greatly simplify use of the fluorophore. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 14% |
| United Kingdom | 1 | 14% |
| Japan | 1 | 14% |
| Bolivia, Plurinational State of | 1 | 14% |
| Korea, Republic of | 1 | 14% |
| Unknown | 2 | 29% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 57% |
| Members of the public | 3 | 43% |
Mendeley readers
The data shown below were compiled from readership statistics for 131 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 2 | 2% |
| United Kingdom | 1 | <1% |
| France | 1 | <1% |
| Korea, Republic of | 1 | <1% |
| Unknown | 126 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 39 | 30% |
| Researcher | 24 | 18% |
| Student > Doctoral Student | 12 | 9% |
| Professor | 9 | 7% |
| Student > Bachelor | 8 | 6% |
| Other | 29 | 22% |
| Unknown | 10 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 69 | 53% |
| Agricultural and Biological Sciences | 19 | 15% |
| Physics and Astronomy | 10 | 8% |
| Biochemistry, Genetics and Molecular Biology | 7 | 5% |
| Materials Science | 5 | 4% |
| Other | 9 | 7% |
| Unknown | 12 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2016.
All research outputs
#3,940,780
of 22,713,403 outputs
Outputs from Nature Chemistry
#2,022
of 2,999 outputs
Outputs of similar age
#34,087
of 194,452 outputs
Outputs of similar age from Nature Chemistry
#34
of 52 outputs
Altmetric has tracked 22,713,403 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,999 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 35.0. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
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We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.