Title |
Transfer of minimally manipulated CMV-specific T cells from stem cell or third-party donors to treat CMV infection after allo-HSCT
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Published in |
Leukemia, January 2017
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DOI | 10.1038/leu.2017.16 |
Pubmed ID | |
Authors |
M Neuenhahn, J Albrecht, M Odendahl, F Schlott, G Dössinger, M Schiemann, S Lakshmipathi, K Martin, D Bunjes, S Harsdorf, E M Weissinger, H Menzel, M Verbeek, L Uharek, N Kröger, E Wagner, G Kobbe, T Schroeder, M Schmitt, G Held, W Herr, L Germeroth, H Bonig, T Tonn, H Einsele, D H Busch, G U Grigoleit |
Abstract |
Cytomegalovirus (CMV) infection is a common, potentially life-threatening complication following allogeneic hematopoietic stem cell transplantation (allo-HSCT). We assessed prospectively safety and efficacy of stem cell-donor- or third-party-donor-derived CMV-specific T cells for the treatment of persistent CMV infections after allo-HSCT in a phase I/IIa trial. Allo-HSCT patients with drug-refractory CMV infection and lacking virus-specific T cells were treated with a single dose of ex vivo MHC-Streptamer-isolated CMV epitope-specific donor T cells. 44 allo-HSCT patients receiving a T cell-replete (D(+)repl; n=28) or T cell-depleted (D(+)depl; n=16) graft from a CMV seropositive donor were screened for CMV-specific T cell immunity. 8 D(+)depl recipients received adoptive T cell therapy from their stem cell donor. CMV epitope-specific T cells were well supported and became detectable in all treated patients. Complete and partial virological response rates were 62.5 and 25%, respectively. Due to longsome third party donor (TPD) identification, only 8 of 57 CMV-patients transplanted from CMV seronegative donors (D(-)) received antigen-specific T cells from partially HLA-matched TPDs. In all but one, TPD-derived CMV-specific T cells remained undetectable. In summary, adoptive transfer correlated with functional virus-specific T cell reconstitution in D(+)depl patients. Suboptimal HLA-match may counteract expansion of TPD-derived virus-specific T cells in D(-) patients.Leukemia accepted article preview online, 16 January 2017. doi:10.1038/leu.2017.16. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 20% |
Spain | 2 | 20% |
United Kingdom | 1 | 10% |
Unknown | 5 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 40% |
Scientists | 3 | 30% |
Science communicators (journalists, bloggers, editors) | 2 | 20% |
Practitioners (doctors, other healthcare professionals) | 1 | 10% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Czechia | 1 | <1% |
Unknown | 115 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 19 | 16% |
Student > Ph. D. Student | 13 | 11% |
Other | 11 | 9% |
Professor | 8 | 7% |
Student > Master | 7 | 6% |
Other | 25 | 22% |
Unknown | 33 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 34 | 29% |
Immunology and Microbiology | 14 | 12% |
Agricultural and Biological Sciences | 11 | 9% |
Biochemistry, Genetics and Molecular Biology | 7 | 6% |
Engineering | 3 | 3% |
Other | 8 | 7% |
Unknown | 39 | 34% |