Title |
Persistent infection with Crohn’s disease-associated adherent-invasive Escherichia coli leads to chronic inflammation and intestinal fibrosis
|
---|---|
Published in |
Nature Communications, June 2013
|
DOI | 10.1038/ncomms2957 |
Pubmed ID | |
Authors |
Cherrie-Lee N. Small, Sarah A. Reid-Yu, Joseph B. McPhee, Brian K. Coombes |
Abstract |
Crohn's disease is a chronic inflammatory condition of the gastrointestinal tract in which alterations to the bacterial community contribute to disease. Adherent-invasive Escherichia coli are associated with human Crohn's disease; however, their role in intestinal immunopathology is unclear because of the lack of an animal model compatible with chronic timescales. Here we establish chronic adherent-invasive Escherichia coli infection in streptomycin-treated conventional mice (CD1, DBA/2, C3H, 129e and C57BL/6), enabling the study of host response and immunopathology. Adherent-invasive Escherichia coli induces an active T-helper 17 response, heightened levels of proinflammatory cytokines and fibrotic growth factors, with transmural inflammation and fibrosis. Depletion of CD8+ T cells increases caecal bacterial load, pathology and intestinal fibrosis in C57BL/6 mice, suggesting a protective role. Our findings provide evidence that chronic adherent-invasive Escherichia coli infections result in immunopathology similar to that seen in Crohn's disease. With this model, research into the host and bacterial genetics associated with adherent-invasive Escherichia coli-induced disease becomes more widely accessible. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 3 | 33% |
Canada | 1 | 11% |
United States | 1 | 11% |
Unknown | 4 | 44% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 8 | 89% |
Scientists | 1 | 11% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 2% |
United Kingdom | 4 | 2% |
Netherlands | 1 | <1% |
Russia | 1 | <1% |
China | 1 | <1% |
Unknown | 186 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 38 | 19% |
Student > Ph. D. Student | 36 | 18% |
Researcher | 32 | 16% |
Student > Master | 23 | 12% |
Other | 7 | 4% |
Other | 23 | 12% |
Unknown | 38 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 61 | 31% |
Biochemistry, Genetics and Molecular Biology | 35 | 18% |
Immunology and Microbiology | 30 | 15% |
Medicine and Dentistry | 14 | 7% |
Chemistry | 4 | 2% |
Other | 9 | 5% |
Unknown | 44 | 22% |