Title |
Cryogenic optical localization provides 3D protein structure data with Angstrom resolution
|
---|---|
Published in |
Nature Methods, January 2017
|
DOI | 10.1038/nmeth.4141 |
Pubmed ID | |
Authors |
Siegfried Weisenburger, Daniel Boening, Benjamin Schomburg, Karin Giller, Stefan Becker, Christian Griesinger, Vahid Sandoghdar |
Abstract |
We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology. |
X Demographics
The data shown below were collected from the profiles of 25 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 7 | 28% |
France | 2 | 8% |
China | 2 | 8% |
Netherlands | 2 | 8% |
Germany | 2 | 8% |
Belgium | 1 | 4% |
Spain | 1 | 4% |
Italy | 1 | 4% |
Denmark | 1 | 4% |
Other | 0 | 0% |
Unknown | 6 | 24% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 15 | 60% |
Scientists | 7 | 28% |
Science communicators (journalists, bloggers, editors) | 3 | 12% |
Mendeley readers
The data shown below were compiled from readership statistics for 180 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | <1% |
Germany | 1 | <1% |
Unknown | 178 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 50 | 28% |
Researcher | 36 | 20% |
Student > Master | 18 | 10% |
Professor | 16 | 9% |
Student > Doctoral Student | 11 | 6% |
Other | 19 | 11% |
Unknown | 30 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Physics and Astronomy | 44 | 24% |
Chemistry | 28 | 16% |
Biochemistry, Genetics and Molecular Biology | 28 | 16% |
Agricultural and Biological Sciences | 27 | 15% |
Engineering | 15 | 8% |
Other | 8 | 4% |
Unknown | 30 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 38. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 June 2019.
All research outputs
#1,017,771
of 24,593,959 outputs
Outputs from Nature Methods
#1,325
of 5,229 outputs
Outputs of similar age
#22,000
of 430,862 outputs
Outputs of similar age from Nature Methods
#28
of 86 outputs
Altmetric has tracked 24,593,959 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,229 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 35.9. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 430,862 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 86 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.