Title |
Mechanism of early dissemination and metastasis in Her2+ mammary cancer
|
---|---|
Published in |
Nature, December 2016
|
DOI | 10.1038/nature20609 |
Pubmed ID | |
Authors |
Kathryn L. Harper, Maria Soledad Sosa, David Entenberg, Hedayatollah Hosseini, Julie F. Cheung, Rita Nobre, Alvaro Avivar-Valderas, Chandandaneep Nagi, Nomeda Girnius, Roger J. Davis, Eduardo F. Farias, John Condeelis, Christoph A. Klein, Julio A. Aguirre-Ghiso |
Abstract |
Metastasis is the leading cause of cancer-related deaths; metastatic lesions develop from disseminated cancer cells (DCCs) that can remain dormant. Metastasis-initiating cells are thought to originate from a subpopulation present in progressed, invasive tumours. However, DCCs detected in patients before the manifestation of breast-cancer metastasis contain fewer genetic abnormalities than primary tumours or than DCCs from patients with metastases. These findings, and those in pancreatic cancer and melanoma models, indicate that dissemination might occur during the early stages of tumour evolution. However, the mechanisms that might allow early disseminated cancer cells (eDCCs) to complete all steps of metastasis are unknown. Here we show that, in early lesions in mice and before any apparent primary tumour masses are detected, there is a sub-population of Her2(+)p-p38(lo)p-Atf2(lo)Twist1(hi)E-cad(lo) early cancer cells that is invasive and can spread to target organs. Intra-vital imaging and organoid studies of early lesions showed that Her2(+) eDCC precursors invaded locally, intravasated and lodged in target organs. Her2(+) eDCCs activated a Wnt-dependent epithelial-mesenchymal transition (EMT)-like dissemination program but without complete loss of the epithelial phenotype, which was reversed by Her2 or Wnt inhibition. Notably, although the majority of eDCCs were Twist1(hi)E-cad(lo) and dormant, they eventually initiated metastasis. Our work identifies a mechanism for early dissemination in which Her2 aberrantly activates a program similar to mammary ductal branching that generates eDCCs that are capable of forming metastasis after a dormancy phase. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 24 | 25% |
United Kingdom | 9 | 9% |
Italy | 5 | 5% |
Australia | 3 | 3% |
France | 3 | 3% |
Peru | 2 | 2% |
Mexico | 2 | 2% |
Spain | 2 | 2% |
India | 2 | 2% |
Other | 10 | 10% |
Unknown | 35 | 36% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 59 | 61% |
Scientists | 29 | 30% |
Science communicators (journalists, bloggers, editors) | 5 | 5% |
Practitioners (doctors, other healthcare professionals) | 4 | 4% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | <1% |
United Kingdom | 3 | <1% |
France | 1 | <1% |
Italy | 1 | <1% |
Netherlands | 1 | <1% |
Germany | 1 | <1% |
Australia | 1 | <1% |
Japan | 1 | <1% |
Mexico | 1 | <1% |
Other | 0 | 0% |
Unknown | 609 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 152 | 24% |
Researcher | 123 | 20% |
Student > Master | 63 | 10% |
Student > Bachelor | 46 | 7% |
Student > Doctoral Student | 39 | 6% |
Other | 100 | 16% |
Unknown | 102 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 181 | 29% |
Agricultural and Biological Sciences | 133 | 21% |
Medicine and Dentistry | 95 | 15% |
Immunology and Microbiology | 21 | 3% |
Engineering | 15 | 2% |
Other | 62 | 10% |
Unknown | 118 | 19% |