| Title |
Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions
|
|---|---|
| Published in |
Nature Biomedical Engineering, December 2016
|
| DOI | 10.1038/s41551-016-0003 |
| Pubmed ID | |
| Authors |
Xiaoping Bao, Xiaojun Lian, Timothy A. Hacker, Eric G. Schmuck, Tongcheng Qian, Vijesh J. Bhute, Tianxiao Han, Mengxuan Shi, Lauren Drowley, Alleyn T. Plowright, Qing-Dong Wang, Marie-Jose Goumans, Sean P. Palecek |
| Abstract |
The epicardium contributes both multi-lineage descendants and paracrine factors to the heart during cardiogenesis and cardiac repair, underscoring its potential for cardiac regenerative medicine. Yet little is known about the cellular and molecular mechanisms that regulate human epicardial development and regeneration. Here, we show that the temporal modulation of canonical Wnt signaling is sufficient for epicardial induction from 6 different human pluripotent stem cell (hPSC) lines, including a WT1-2A-eGFP knock-in reporter line, under chemically-defined, xeno-free conditions. We also show that treatment with transforming growth factor beta (TGF-β)-signalling inhibitors permitted long-term expansion of the hPSC-derived epicardial cells, resulting in a more than 25 population doublings of WT1+ cells in homogenous monolayers. The hPSC-derived epicardial cells were similar to primary epicardial cells both in vitro and in vivo, as determined by morphological and functional assays, including RNA-seq. Our findings have implications for the understanding of self-renewal mechanisms of the epicardium and for epicardial regeneration using cellular or small-molecule therapies. |
X Demographics
The data shown below were collected from the profiles of 28 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 18% |
| United Kingdom | 2 | 7% |
| Italy | 1 | 4% |
| Australia | 1 | 4% |
| Singapore | 1 | 4% |
| Colombia | 1 | 4% |
| Greece | 1 | 4% |
| Unknown | 16 | 57% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 15 | 54% |
| Scientists | 10 | 36% |
| Science communicators (journalists, bloggers, editors) | 2 | 7% |
| Practitioners (doctors, other healthcare professionals) | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 146 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 146 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 38 | 26% |
| Researcher | 22 | 15% |
| Student > Master | 15 | 10% |
| Student > Bachelor | 14 | 10% |
| Professor > Associate Professor | 7 | 5% |
| Other | 23 | 16% |
| Unknown | 27 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 30 | 21% |
| Agricultural and Biological Sciences | 24 | 16% |
| Engineering | 23 | 16% |
| Medicine and Dentistry | 14 | 10% |
| Chemical Engineering | 5 | 3% |
| Other | 17 | 12% |
| Unknown | 33 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 170. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 December 2022.
All research outputs
#233,713
of 25,048,615 outputs
Outputs from Nature Biomedical Engineering
#137
of 1,116 outputs
Outputs of similar age
#4,902
of 427,580 outputs
Outputs of similar age from Nature Biomedical Engineering
#3
of 23 outputs
Altmetric has tracked 25,048,615 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,116 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 87.4. This one has done well, scoring higher than 87% of its peers.
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We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.