| Title |
Neutralization mechanism of a highly potent antibody against Zika virus
|
|---|---|
| Published in |
Nature Communications, November 2016
|
| DOI | 10.1038/ncomms13679 |
| Pubmed ID | |
| Authors |
Shuijun Zhang, Victor A. Kostyuchenko, Thiam-Seng Ng, Xin-Ni Lim, Justin S. G. Ooi, Sebastian Lambert, Ter Yong Tan, Douglas G. Widman, Jian Shi, Ralph S. Baric, Shee-Mei Lok |
| Abstract |
The rapid spread of Zika virus (ZIKV), which causes microcephaly and Guillain-Barré syndrome, signals an urgency to identify therapeutics. Recent efforts to rescreen dengue virus human antibodies for ZIKV cross-neutralization activity showed antibody C10 as one of the most potent. To investigate the ability of the antibody to block fusion, we determined the cryoEM structures of the C10-ZIKV complex at pH levels mimicking the extracellular (pH8.0), early (pH6.5) and late endosomal (pH5.0) environments. The 4.0 Å resolution pH8.0 complex structure shows that the antibody binds to E proteins residues at the intra-dimer interface, and the virus quaternary structure-dependent inter-dimer and inter-raft interfaces. At pH6.5, antibody C10 locks all virus surface E proteins, and at pH5.0, it locks the E protein raft structure, suggesting that it prevents the structural rearrangement of the E proteins during the fusion event-a vital step for infection. This suggests antibody C10 could be a good therapeutic candidate. |
X Demographics
The data shown below were collected from the profiles of 28 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 8 | 29% |
| France | 2 | 7% |
| Spain | 2 | 7% |
| Netherlands | 2 | 7% |
| Ecuador | 1 | 4% |
| Hungary | 1 | 4% |
| Mexico | 1 | 4% |
| Italy | 1 | 4% |
| Unknown | 10 | 36% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 18 | 64% |
| Scientists | 9 | 32% |
| Practitioners (doctors, other healthcare professionals) | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 167 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 167 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 32 | 19% |
| Researcher | 30 | 18% |
| Student > Master | 26 | 16% |
| Student > Bachelor | 25 | 15% |
| Professor > Associate Professor | 6 | 4% |
| Other | 18 | 11% |
| Unknown | 30 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 42 | 25% |
| Immunology and Microbiology | 27 | 16% |
| Agricultural and Biological Sciences | 22 | 13% |
| Medicine and Dentistry | 11 | 7% |
| Chemistry | 8 | 5% |
| Other | 23 | 14% |
| Unknown | 34 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 209. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2017.
All research outputs
#164,512
of 23,576,969 outputs
Outputs from Nature Communications
#2,308
of 49,146 outputs
Outputs of similar age
#3,747
of 418,392 outputs
Outputs of similar age from Nature Communications
#53
of 812 outputs
Altmetric has tracked 23,576,969 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 49,146 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 56.2. This one has done particularly well, scoring higher than 95% of its peers.
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We're also able to compare this research output to 812 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.