| Title |
Whole-brain functional imaging at cellular resolution using light-sheet microscopy
|
|---|---|
| Published in |
Nature Methods, March 2013
|
| DOI | 10.1038/nmeth.2434 |
| Pubmed ID | |
| Authors |
Misha B Ahrens, Michael B Orger, Drew N Robson, Jennifer M Li, Philipp J Keller |
| Abstract |
Brain function relies on communication between large populations of neurons across multiple brain areas, a full understanding of which would require knowledge of the time-varying activity of all neurons in the central nervous system. Here we use light-sheet microscopy to record activity, reported through the genetically encoded calcium indicator GCaMP5G, from the entire volume of the brain of the larval zebrafish in vivo at 0.8 Hz, capturing more than 80% of all neurons at single-cell resolution. Demonstrating how this technique can be used to reveal functionally defined circuits across the brain, we identify two populations of neurons with correlated activity patterns. One circuit consists of hindbrain neurons functionally coupled to spinal cord neuropil. The other consists of an anatomically symmetric population in the anterior hindbrain, with activity in the left and right halves oscillating in antiphase, on a timescale of 20 s, and coupled to equally slow oscillations in the inferior olive. |
X Demographics
The data shown below were collected from the profiles of 357 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 68 | 19% |
| Japan | 33 | 9% |
| United Kingdom | 24 | 7% |
| Canada | 18 | 5% |
| Spain | 12 | 3% |
| Australia | 7 | 2% |
| France | 6 | 2% |
| Germany | 5 | 1% |
| Mexico | 5 | 1% |
| Other | 45 | 13% |
| Unknown | 134 | 38% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 246 | 69% |
| Scientists | 90 | 25% |
| Science communicators (journalists, bloggers, editors) | 13 | 4% |
| Practitioners (doctors, other healthcare professionals) | 8 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,991 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 49 | 2% |
| Germany | 19 | <1% |
| United Kingdom | 13 | <1% |
| Japan | 9 | <1% |
| China | 8 | <1% |
| Spain | 7 | <1% |
| Netherlands | 6 | <1% |
| Portugal | 6 | <1% |
| France | 5 | <1% |
| Other | 33 | 2% |
| Unknown | 1836 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 566 | 28% |
| Researcher | 410 | 21% |
| Student > Master | 190 | 10% |
| Student > Bachelor | 162 | 8% |
| Student > Doctoral Student | 98 | 5% |
| Other | 308 | 15% |
| Unknown | 257 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 589 | 30% |
| Neuroscience | 331 | 17% |
| Physics and Astronomy | 190 | 10% |
| Engineering | 188 | 9% |
| Biochemistry, Genetics and Molecular Biology | 132 | 7% |
| Other | 267 | 13% |
| Unknown | 294 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 661. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2022.
All research outputs
#33,090
of 25,770,491 outputs
Outputs from Nature Methods
#6
of 5,414 outputs
Outputs of similar age
#155
of 223,581 outputs
Outputs of similar age from Nature Methods
#1
of 71 outputs
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