| Title |
Zymography methods for visualizing hydrolytic enzymes
|
|---|---|
| Published in |
Nature Methods, February 2013
|
| DOI | 10.1038/nmeth.2371 |
| Pubmed ID | |
| Authors |
Jennifer Vandooren, Nathalie Geurts, Erik Martens, Philippe E Van den Steen, Ghislain Opdenakker |
| Abstract |
Zymography is a technique for studying hydrolytic enzymes on the basis of substrate degradation. It is a powerful, but often misinterpreted, tool yielding information on potential hydrolytic activities, enzyme forms and the locations of active enzymes. In this Review, zymography techniques are compared in terms of advantages, limitations and interpretations. With in gel zymography, enzyme forms are visualized according to their molecular weights. Proteolytic activities are localized in tissue sections with in situ zymography. In vivo zymography can pinpoint proteolytic activity to sites in an intact organism. Future development of novel substrate probes and improvement in detection and imaging methods will increase the applicability of zymography for (reverse) degradomics studies. |
Mendeley readers
The data shown below were compiled from readership statistics for 645 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | <1% |
| India | 3 | <1% |
| Australia | 3 | <1% |
| Chile | 2 | <1% |
| Ireland | 1 | <1% |
| France | 1 | <1% |
| Colombia | 1 | <1% |
| Netherlands | 1 | <1% |
| Israel | 1 | <1% |
| Other | 2 | <1% |
| Unknown | 626 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 150 | 23% |
| Student > Master | 116 | 18% |
| Researcher | 87 | 13% |
| Student > Bachelor | 85 | 13% |
| Student > Doctoral Student | 34 | 5% |
| Other | 83 | 13% |
| Unknown | 90 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 214 | 33% |
| Biochemistry, Genetics and Molecular Biology | 145 | 22% |
| Medicine and Dentistry | 47 | 7% |
| Chemistry | 30 | 5% |
| Immunology and Microbiology | 23 | 4% |
| Other | 85 | 13% |
| Unknown | 101 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 January 2024.
All research outputs
#4,861,321
of 23,408,972 outputs
Outputs from Nature Methods
#3,135
of 5,010 outputs
Outputs of similar age
#40,364
of 194,533 outputs
Outputs of similar age from Nature Methods
#45
of 68 outputs
Altmetric has tracked 23,408,972 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,010 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 35.2. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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We're also able to compare this research output to 68 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.