| Title |
Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes
|
|---|---|
| Published in |
Nature Communications, September 2016
|
| DOI | 10.1038/ncomms12676 |
| Pubmed ID | |
| Authors |
Toru Suzuki, Maki Asami, Martin Hoffmann, Xin Lu, Miodrag Gužvić, Christoph A. Klein, Anthony C. F. Perry |
| Abstract |
Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for full-term development. Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm developed to produce healthy offspring at up to 24% of control rates, depending when in the embryonic cell cycle injection took place. This implies that most of the first embryonic cell cycle can be bypassed in sperm genome reprogramming for full development. Remodelling of histones and genomic 5'-methylcytosine and 5'-hydroxymethylcytosine following embryo injection were distinct from remodelling in fertilization and the resulting 2-cell embryos consistently possessed abnormal transcriptomes. These studies demonstrate plasticity in the reprogramming of terminally differentiated sperm nuclei and suggest that different epigenetic pathways or kinetics can establish totipotency. |
X Demographics
The data shown below were collected from the profiles of 157 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 17 | 11% |
| United States | 15 | 10% |
| United Kingdom | 8 | 5% |
| France | 7 | 4% |
| Spain | 6 | 4% |
| Peru | 3 | 2% |
| Saudi Arabia | 3 | 2% |
| Canada | 3 | 2% |
| Germany | 3 | 2% |
| Other | 18 | 11% |
| Unknown | 74 | 47% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 132 | 84% |
| Scientists | 16 | 10% |
| Practitioners (doctors, other healthcare professionals) | 5 | 3% |
| Science communicators (journalists, bloggers, editors) | 4 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 188 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 1% |
| Brazil | 2 | 1% |
| Germany | 1 | <1% |
| Netherlands | 1 | <1% |
| Belgium | 1 | <1% |
| Mexico | 1 | <1% |
| Unknown | 180 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 40 | 21% |
| Student > Postgraduate | 33 | 18% |
| Student > Bachelor | 26 | 14% |
| Student > Master | 23 | 12% |
| Student > Ph. D. Student | 20 | 11% |
| Other | 26 | 14% |
| Unknown | 20 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 67 | 36% |
| Agricultural and Biological Sciences | 63 | 34% |
| Medicine and Dentistry | 12 | 6% |
| Engineering | 6 | 3% |
| Neuroscience | 4 | 2% |
| Other | 14 | 7% |
| Unknown | 22 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 977. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 January 2024.
All research outputs
#17,038
of 25,715,849 outputs
Outputs from Nature Communications
#333
of 58,190 outputs
Outputs of similar age
#253
of 331,978 outputs
Outputs of similar age from Nature Communications
#8
of 863 outputs
Altmetric has tracked 25,715,849 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 58,190 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.4. This one has done particularly well, scoring higher than 99% of its peers.
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We're also able to compare this research output to 863 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.