| Title |
Analyzing membrane remodeling and fission using supported bilayers with excess membrane reservoir
|
|---|---|
| Published in |
Nature Protocols, January 2013
|
| DOI | 10.1038/nprot.2012.152 |
| Pubmed ID | |
| Authors |
Sylvia Neumann, Thomas J Pucadyil, Sandra L Schmid |
| Abstract |
A complete understanding of the molecular mechanisms governing vesicle formation requires quantitative assays and vesicle reconstitution using purified components. We describe a simple model membrane template for studying protein-mediated membrane remodeling and vesicle formation or fission that is amenable to both quantitative biochemical analysis and real-time imaging by epifluorescence microscopy. Supported bilayers with excess membrane reservoir (SUPER) templates are compositionally well-defined unilamellar membrane systems prepared on 2-5-μm silica beads under conditions that enable incorporation of excess membrane to form a loosely fitting bilayer that can be used to study membrane remodeling and fission. This protocol describes methods for SUPER template formation and characterization, as well as for the qualitative observation and quantitative measurement of vesicle formation and fission via microscopy and a simple sedimentation assay. SUPER templates can be prepared within 60 min. Results from either sedimentation-based or microscopy-based assays can be obtained within an additional 60 min. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 1% |
| United States | 1 | 1% |
| France | 1 | 1% |
| Korea, Republic of | 1 | 1% |
| Unknown | 65 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 26% |
| Researcher | 13 | 19% |
| Student > Master | 6 | 9% |
| Student > Bachelor | 5 | 7% |
| Student > Doctoral Student | 5 | 7% |
| Other | 11 | 16% |
| Unknown | 11 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 27 | 39% |
| Biochemistry, Genetics and Molecular Biology | 18 | 26% |
| Chemistry | 5 | 7% |
| Engineering | 2 | 3% |
| Neuroscience | 2 | 3% |
| Other | 5 | 7% |
| Unknown | 10 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 January 2013.
All research outputs
#13,375,146
of 22,691,736 outputs
Outputs from Nature Protocols
#2,345
of 2,727 outputs
Outputs of similar age
#158,231
of 280,814 outputs
Outputs of similar age from Nature Protocols
#29
of 34 outputs
Altmetric has tracked 22,691,736 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
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