| Title |
Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli
|
|---|---|
| Published in |
Nature Communications, August 2016
|
| DOI | 10.1038/ncomms12568 |
| Pubmed ID | |
| Authors |
Mathew Stracy, Marcin Jaciuk, Stephan Uphoff, Achillefs N. Kapanidis, Marcin Nowotny, David J. Sherratt, Pawel Zawadzki |
| Abstract |
Nucleotide excision repair (NER) removes chemically diverse DNA lesions in all domains of life. In Escherichia coli, UvrA and UvrB initiate NER, although the mechanistic details of how this occurs in vivo remain to be established. Here, we use single-molecule fluorescence imaging to provide a comprehensive characterization of the lesion search, recognition and verification process in living cells. We show that NER initiation involves a two-step mechanism in which UvrA scans the genome and locates DNA damage independently of UvrB. Then UvrA recruits UvrB from solution to the lesion. These steps are coordinated by ATP binding and hydrolysis in the 'proximal' and 'distal' UvrA ATP-binding sites. We show that initial UvrB-independent damage recognition by UvrA requires ATPase activity in the distal site only. Subsequent UvrB recruitment requires ATP hydrolysis in the proximal site. Finally, UvrA dissociates from the lesion complex, allowing UvrB to orchestrate the downstream NER reactions. |
Mendeley readers
The data shown below were compiled from readership statistics for 132 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| United Kingdom | 1 | <1% |
| Unknown | 129 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 42 | 32% |
| Student > Bachelor | 21 | 16% |
| Researcher | 15 | 11% |
| Student > Master | 8 | 6% |
| Student > Doctoral Student | 7 | 5% |
| Other | 14 | 11% |
| Unknown | 25 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 61 | 46% |
| Agricultural and Biological Sciences | 25 | 19% |
| Chemistry | 7 | 5% |
| Immunology and Microbiology | 4 | 3% |
| Physics and Astronomy | 4 | 3% |
| Other | 7 | 5% |
| Unknown | 24 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2016.
All research outputs
#13,903,378
of 23,577,761 outputs
Outputs from Nature Communications
#40,950
of 49,102 outputs
Outputs of similar age
#183,643
of 340,836 outputs
Outputs of similar age from Nature Communications
#706
of 862 outputs
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